Flaviviridae, Flavivirus, Dengue Virus
  Serotypes 1-4
The information provided below is for MIT researchers' use and is subject to change by EHS. Pleaese see our Legal disclaimer
Agent Characteristics
Virus
RG 2
Humans;NHP;Mosquitos
Cut/ Needlestick / Non-intact skin;Mosquito bite;Mother to fetus transmission;Mucous Membranes
Dengue Virus is an enveloped, positive-sense RNA virus of the Flaviviridae family and Flavivirus genus. In nature it is transmitted to human by the bite of an infected mosquito and causes a disease known as dengue fever or breakbone fever. There are four serotype of the virus and re-infection with different serotypes can increase the severity of disease.
Infectious Dose: Less than 10. Incubation period of 3 to 14 days; usually 4 to 7 days.
Health Hazards
Fever, headache, ocular pain, myalgias (body aches), rash, and bleeding disorders. Can lead to shock.
Dengvaxia is a vaccine licensed in over 20 countries.
Prophylaxis targets personal protection from mosquito bites
No current antiviral therapy is available and therefore treatment is supportive care.
Containment
BSL 2 ABSL2
PPE/Containment
Wear gloves, lab coat, and appropriate lab attire (pants/long dress, closed toed shoes); eye protection, e.g. safety glasses, safety goggles recommended for liquids. Additional PPE may be required depending on lab specific SOPs for handling Flaviviridae, Flavivirus, Dengue Virus.
Biosafety cabinet recommended for procedures that can generate aerosols or splashes, Minimize use of sharps
Viability
Moist heat (121°C for at least 15 min), dry heat (160-170°C for at least 1 hour)
10% household bleach (~0.5% sodium hypochlorite);70% ethanol;Activated hydrogen peroxide (PREempt, Rescue);Quaternary ammonia (Quatricide, sklar, Lysol spray, etc.);Phenolic (Vesphene II);hydrogen peroxide and peracetic acid (Peridox);Fresh 2% glutaraldehyde (Gluterate / CIDEX)
Can survive several days in dried blood at room temp
Spill Procedures
Flush eyes, mouth, or nose for 15 minutes at eyewash station.
Wash area with soap and water and rinse/scrub for 5-10 minutes.
Immediately report incident to supervisor, PI, or EHS Representative. PI/supervisor must submit incident report in Atlas within 24 hours.
Notify others working in the lab.  Allow aerosols to settle. Don appropriate PPE.  Cover area of the spill with paper towels and apply an EPA approved disinfectant, working from the perimeter towards the center.  Allow 10 minutes of contact time. Cleanup spill materials and dispose as biowaste. Surface disinfect 5-10 minutes. Remove PPE and wash hands.
For large volume spills or biological spills in a public area, contact MIT's Biosafety Program or the EHS office (617-452-3477) during normal business hours.  If the spill occurs after hours or on weekends, activate the EHS ON CALL system by dialing Operations 3-4948 (617-253-4948) or MIT Police by dialing 100 from a campus phone or 617-253-1212 from a mobile phone.
Medical Follow-up
9:00am-4:00pm, weekdays:
contact MIT Occupational Health:
Phone: 617-253-8552
Address: 25 Carleton Street, Building E23, Cambridge, MA 02142
website		 8:00am-8:00pm, weekdays
10:00am-4:00pm, weekends
MIT Health Urgent Care
phone: 617-253-1311
address: 25 Carleton Street, Building E23, Cambridge, MA 02142
Or call MIT Police 617-253-1212		 Emergencies:
Call 100 from a campus phone or
617-253-1212 from a mobile phone
Additional comments
All Category A Infectious agents solid waste, e.g petri dishes, tubes, contaminated gloves, must be inactivated with an appropriate disinfectant before disposing into the biowaste box
References
Show fields from Show fields from Show fields from a related table
Report Name *
Description
Reports and Charts Panel
Each table has a panel listing its reports and charts, organized in groups.
Please wait while your new report is saved...
Field label
Column heading override
Justification
What does auto mean?
Fields in:

Fields to Extract:
Name for the new table:
Items in the new table are called:

When you bring additional fields into a conversion, Quickbase often finds inconsistencies. For example, say you're converting your Companies column into its own table. One company, Acme Corporation, has offices in New York, Dallas and Portland. So, when you add the City column to the conversion, Quickbase finds three different locations for Acme. A single value in the column you're converting can only match one value in any additional field. Quickbase needs you to clean up the extra cities before it can create your new table. To do so, you have one of two choices:

  • If you want to create three separate Acme records (Acme-New York, Acme-Dallas and Acme-Portland) click the Conform link at the top of the column.
  • If the dissimilar entries are mistakes (say Acme only has one office in New York and the other locations are data-entry errors) go back into your table and correct the inconsistencies—in this case, changing all locations to New York. Then try the conversion again.

Read more about converting a column into a table.