Researcher Information
Sharoni Michal
sharonim@mit.edu (123) 456-7890
The information provided below is for MIT researchers' use and is subject to change by EHS. For our legal disclaimer please see - http://ehs.mit.edu/site/content/legal-disclaimer
Agent Characteristics
Shigella spp.
Shiga toxin producing strains
RG 2
Bacteria
Yes       Yes    
Family Enterobacteriaceae, Gram-negative rod-shaped non-motile pathogenic bacteria. Facultative anaerobes
HumansPrimates
Cut/ Needlestick / Non-intact skinIngestionMucous Membranes
Infectious dose: As few as 10 bacteria. Incubation time: Typically 1-3 days, maybe up to 7 days
Health Hazards
Symptoms vary from watery diarrhea only, to more severe illness with high fever, abdominal pain/cramps, rectal pain, mucoid (possibly bloody) stools, perforated large intestines, and bloodstream infections, among others. 
None available
None available
Oral rehydration with added electrolytes is preferred, though IV rehydration may be needed. A short course of antibiotics is often required (ciprofloxacin, azithromycin, ampicillin, Bactrim, IV ceftriaxone) but may not decrease infectivity. Do not use antidiarrheal medications (e.g. loperamide), as they can prolong disease and increase the risk of complications.
Containment
BSL 2
ABSL2
Viability
10% household bleach (~0.5% sodium hypochlorite)70% ethanolActivated hydrogen peroxide (PREempt, Rescue)Quaternary ammonia (Quatricide, sklar, Lysol spray, etc.)Phenolic (Vesphene II)hydrogen peroxide and peracetic acid (Peridox)
Autoclave at 121°C for 30 minutes
Can survive up to months on dry inanimate surfaces.
PPE/Containment
Wear gloves, lab coat, and appropriate lab attire (pants/long dress, closed toed shoes); eye protection, e.g. safety glasses, safety goggles recommended for liquids. Additional PPE may be required depending on lab specific SOPs for handling Shigella spp..
Biosafety cabinet recommended for procedures that can generate aerosols or splashes, Minimize use of sharps
Spill Procedures
Notify others working in the lab.  Allow aerosols to settle. Don appropriate PPE.  Cover area of the spill with paper towels and apply an EPA approved disinfectant, working from the perimeter towards the center.  Allow 10 minutes of contact time. Cleanup spill materials and dispose as biowaste. Surface disinfect 5-10 minutes. Remove PPE and wash hands.
For large volume spills or biological spills in a public area, contact MIT's Biosafety Program or the EHS office (617-452-3477) during normal business hours.  If the spill occurs after hours or on weekends, activate the EHS ON CALL system by dialing Operations 3-4948 (617-253-4948) or MIT Police by dialing 100 from a campus phone or 617-253-1212 from a mobile phone.
Exposure Procedures
Flush eyes, mouth, or nose for 15 minutes at eyewash station.
Wash area with soap and water and rinse/scrub for 5-10 minutes.
Immediately report incident to supervisor, PI, or EHS Representative. PI/supervisor must submit incident report in Atlas within 24 hours.
Medical Follow-up
9:00am-4:00pm, weekdays:
contact MIT Occupational Health:
Phone: 617-253-8552
Address: 25 Carleton Street, Building E23, Cambridge, MA 02142
website		 8:00am-8:00pm, weekdays
10:00am-4:00pm, weekends
MIT Health Urgent Care
phone: 617-253-1311
address: 25 Carleton Street, Building E23, Cambridge, MA 02142
Or call MIT Police 617-253-1212		 Emergencies:
Call 100 from a campus phone or
617-253-1212 from a mobile phone
Additional comments
All Category A Infectious agents solid waste, e.g petri dishes, tubes, contaminated gloves, must be inactivated with an appropriate disinfectant before disposing into the biowaste box
References
Created on Oct. 18, 2021 at  4:32 PM (EDT). Last updated by Zhong, Lu on Nov. 19, 2025 at  5:23 PM (EST). Owned by Sharoni, Michal.
Michal Sharoni
Lu Zhong
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