Researcher Information
Sharoni Michal
sharonim@mit.edu (123) 456-7890
The information provided below is for MIT researchers' use and is subject to change by EHS. For our legal disclaimer please see - http://ehs.mit.edu/site/content/legal-disclaimer
Agent Characteristics
Burkholderia cenocepacia
RG 2
Bacteria
Yes   Yes        
Gram-negative member of the Burkholderia cepacia  complex (BCC) are widespread in the environment.  Multidrug resistant and may cause disease in plants.  
HumansAnimalsPlants
Cut/ Needlestick / Non-intact skinMucous Membranes
Infectious dose: Unknown.Incubation time: Unknown for B cenocepacia.  The incubation period for a related pathogen, B pseudomallei, is 1-21 days but may be years. 
Health Hazards
Opportunistic pathogen, primarily affecting individuals with weakened immune systems or chronic lung disease, such as cystic fibrosis (CF).  May cause pneumonia with low-grade fever, fluid around the lungs, or lung abscesses.  Known to spread person-to-person in those with CF.   May also spread via contaminated pharmaceutical products. 
None available
None available
Antibiotic therapy (e.g. meropenem, ceftazadime, doxycycline, etc.) is available, but may require multiple antibiotics taken together.  Intrinsically resistant to aminoglycosides and polymyxins. 
Containment
BSL 2
ABSL2
Viability
10% household bleach (~0.5% sodium hypochlorite)70% ethanolActivated hydrogen peroxide (PREempt, Rescue)Quaternary ammonia (Quatricide, sklar, Lysol spray, etc.)Phenolic (Vesphene II)hydrogen peroxide and peracetic acid (Peridox)Fresh 2% glutaraldehyde (Gluterate / CIDEX)
Autoclave at 121°C for 30 minutes or dry heat 160-170°C for 1-2 hours.
Does not survive on dry surfaces for more than a week. can survive months to years in water.
Accidental injection, direct contact with mucous membraine or non-intact skin, or ingestion
PPE/Containment
Wear gloves, lab coat, and appropriate lab attire (pants/long dress, closed toed shoes); eye protection, e.g. safety glasses, safety goggles recommended for liquids. Additional PPE may be required depending on lab specific SOPs for handling Burkholderia cenocepacia.
Biosafety cabinet recommended for procedures that can generate aerosols or splashes, Minimize use of sharps
Spill Procedures
Notify others working in the lab.  Allow aerosols to settle. Don appropriate PPE.  Cover area of the spill with paper towels and apply an EPA approved disinfectant, working from the perimeter towards the center.  Allow 10 minutes of contact time. Cleanup spill materials and dispose as biowaste. Surface disinfect 5-10 minutes. Remove PPE and wash hands.
For large volume spills or biological spills in a public area, contact MIT's Biosafety Program or the EHS office (617-452-3477) during normal business hours.  If the spill occurs after hours or on weekends, activate the EHS ON CALL system by dialing Operations 3-4948 (617-253-4948) or MIT Police by dialing 100 from a campus phone or 617-253-1212 from a mobile phone.
Exposure Procedures
Flush eyes, mouth, or nose for 15 minutes at eyewash station.
Wash area with soap and water and rinse/scrub for 5-10 minutes.
Immediately report incident to supervisor, PI, or EHS Representative. PI/supervisor must submit incident report in Atlas within 24 hours.
Medical Follow-up
9:00am-4:00pm, weekdays:
contact MIT Occupational Health:
Phone: 617-253-8552
Address: 25 Carleton Street, Building E23, Cambridge, MA 02142
website		 8:00am-8:00pm, weekdays
10:00am-4:00pm, weekends
MIT Health Urgent Care
phone: 617-253-1311
address: 25 Carleton Street, Building E23, Cambridge, MA 02142
Or call MIT Police 617-253-1212		 Emergencies:
Call 100 from a campus phone or
617-253-1212 from a mobile phone
Additional comments
References
Created on April  5, 2021 at 11:56 AM (EDT). Last updated by Zhong, Lu on Nov. 19, 2025 at  5:28 PM (EST). Owned by Zhong, Lu.
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